Composite

Part:BBa_K5317009:Design

Designed by: Jan Gelhoet   Group: iGEM24_Hannover   (2024-09-14)


4xMREa-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To increase promoter efficiency we chose to insert four MREa sites into the promoter sequence and placed them similarly to the physiological MRE sites to prevent interfering with MTF-1 and MRE site interaction.


Source

The promoter was synthesized.

References